PCR as a Confirmatory Technique for Laboratory Diagnosis of Malaria

PCR as a confirmatory technique for laboratory diagnosis of malaria.

We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal.

A new laboratory technique for malaria diagnosis.

Laboratory diagnosis methods of malaria infection

Background and Aim: To manage and control malaria - one of the most important infectious diseases in tropical and subtropical regions – a quick and accurate malaria diagnosis method is necessary as a high priority. For this purpose, several methods have been developed for the diagnosis of malaria, and currently, the examining the peripheral blood slide with an optical microscope as a gold stand...

Laboratory Diagnosis of Malaria

R apid and accurate diagnosis is a key to the success of an effective treatment of malaria. The global impact of malaria has spurred interest in developing diagnostic strategies that will be effective not only in resource-limited areas but also in developed countries, where expertise in malaria diagnosis is often lacking. Accurate diagnosis of malaria is necessary to prevent morbidity and morta...

Novel nested direct PCR technique for malaria diagnosis using filter paper samples.

The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.